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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Analysis, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer program microcal peaq itc analysis software v1 41  (Malvern Panalytical)
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Program Microcal Peaq Itc Analysis Software V1 41, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer assisted sperm analysis casa software  (Hamilton Thorne Ltd)
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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computer-assisted sperm analysis system sperm class analyzer (sca® casa, software version 4.0)  (Microptic SL)
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
Computer Assisted Sperm Analysis System Sperm Class Analyzer (Sca® Casa, Software Version 4.0), supplied by Microptic SL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

Journal: Cells

Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

doi: 10.3390/cells15040360

Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

Techniques: Immunodetection, TUNEL Assay